Impact of Wildtype p53 Induced Phosphatase 1 Gene Lacking on Heart Function in Experimental Mice
10.3969/j.issn.1000-3614.2017.08.015
- VernacularTitle:野生型p53诱导的磷酸酶1基因缺失对小鼠心功能的影响
- Author:
Kemei LIU
;
Chen LIU
;
Peng ZHOU
;
Juan LIU
;
Yu TAN
;
Jiannan LI
;
Lianfeng ZHANG
;
Hongbing ZHANG
;
Hongbing YAN
- Keywords:
Mice;
Gene knockout;
Heart function test;
Apoptosis
- From:
Chinese Circulation Journal
2017;32(8):792-796
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the impact of knocking out wildtype p53 phosphatase 1 gene on heart function with the changes of cardiac tissue mRNA and protein expressions in experimental mice. Methods: Our research included in 2 groups: Wildtype (WT) mice group and Wip1 knockout (Wip1-KO) mice group. n=10 in each group. The heart function, ratio of heart weight to body weight (HW/BW) were examined and compared between 2 groups; cardiac tissue morphology was observed by HE staining; mRNA expressions of ANP, BNP, MCP-1 andα-SMA were determined by RT-PCR and protein expressions of Bcl-2, Bax and c-caspase3 were measured by Western blot analysis. Results: Compared with WT mice group, Wip1-KO mice group showed decreased Wip1 mRNA expression,P<0.05, decreased LVEF, LV fraction shortening and increased left ventricular end systolic diameter (LVESD), allP<0.05; Wip1-KO mice group had reduced BW and elevated ratio of HW/BW, bothP<0.05 even the heart weight was similar between 2 groups. There was no difference in cardiac tissue morphology between 2 groups; mRNA expressions of ANP, BNP, MCP-1 and α-SMA were similar between 2 groups,P>0.05; apoptosis related protein expressions of Bax/Bcl-2 and c-caspase3 were similar between 2 groups,P>0.05. Conclusion: Wip1 gene knockout may impair the heart function in experimental mice, while the relevant mechanism should be further investigated.
