Detection and Typing of Human Papillomavirus in Anal Condyloma Acuminatum of HIV-positive Patients.
10.4174/jkss.2010.78.2.111
- Author:
Suk Bae MOON
1
;
Sang Hee MOON
;
Kyu Joo PARK
Author Information
1. Department of Surgery, Seoul National University College of Medicine, Seoul, Korea. kjparkmd@plaza.snu.ac.kr
- Publication Type:Original Article
- Keywords:
Anal condyloma acuminatum;
Human papillomavirus;
Human immunodeficiency virus;
Polymerase chain reaction;
DNA microarray
- MeSH:
Cervix Uteri;
Chimera;
Coinfection;
DNA;
Female;
HIV;
Humans;
Male;
Oligonucleotide Array Sequence Analysis;
Polymerase Chain Reaction
- From:Journal of the Korean Surgical Society
2010;78(2):111-115
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Anal condyloma is an epithelial proliferative lesion caused by human papillomavirus (HPV) infection. The present study analyzed the HPV types detected in HIV (+) Korean anal condyloma using PCR-based DNA microarray. METHODS: DNA was extracted from the condyloma tissue of 17 patients including 9 HIV (+) patients (M:F=15:2, mean age 35 years, 22~59 years). The 1st PCR was performed with a general primer on L1 region, and nested PCR on the products of the 1st PCR. PCR products were hybridized with a DNA chip. RESULTS: Fourteen patients (9 HIV (+), 5 HIV (-)) showed positive HPV DNA. Overall, type 6 was the most common (N=11), and type 11 (N=6), type 53 (N=3) in order. Among HIV (+) patients, type 6 was also the most common (N=7), then type 11 (N=5) and type 53 (N=3). In contrast to the HIV (-) patients, 5 patients (55.6%) proved to have multiple infections in HIV (+) patients (2 double, 2 triple, 1 quadruple infection). Four of 9 HIV (+) patients (44.5%) showed co-infection with high-risk HPV. CONCLUSION: Multiple infection and co-infection with high-risk types are more prevalent in HIV (+) condyloma patients compared to HIV (-) patients. HPV types on HIV (+) male anal condyloma are similar to those detected in the Korean female uterine cervix.
