Role of M3 receptor in the effect of penehyclidine hydrochloride up-regulating β-arrestin-1 expression
10.3969/j.issn.1671-8348.2017.13.007
- VernacularTitle:盐酸戊乙奎醚上调β抑制蛋白-1与M3受体的关系研究
- Author:
Bin CHENG
;
Yipeng WANG
;
Jia ZHAN
- Keywords:
M3 receptor;
penehyclidine hydrochloride;
β-arrestin-1;
shRNA
- From:
Chongqing Medicine
2017;46(13):1750-1752
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of M3 receptor in the effect of penehyclidine hydrochloride(PHC) upregulating β-arrestin-1 expression in lipopolysaccharide(LPS)-induced human pulmonary microvascular endothelial cell(HPMVEC) injury.Methods.M3 shRNA transfected HPMVEC and normal HPMVEC cells were randomly divided into LPS group(A),LPS+pHC group(B),LPS+ M3 shRNA transfection group(C) and PHC+ LPS+ M3 shRNA transfection group(D).The cytoskeleton change was observed by laser scanning confocal.The LDH level in cellular supernate was detected.The VCAM 1 protein expression was examined by immunofluorescence chemistry.β-arrestin-1 protein expression was determined by Western blot and β-arrestin-1mRNA expression was measured by real-time PCR.Results Compared with the group A or C,F-actin cytoskeleton arrangement in the group B or D was neat,the LDH level and VCAM-1 protein expression were decreased,and β-arrestin-1 expression was increased;compared with group A or B,F-actin cytoskeleton arrangement in the group C or D was neat,the LDH level and VCAM-1 protein expression were decreased,while the β-arrestin-1 expression had no obvious change.Conclusion Silence M3 receptor is conducive to reduce LPS-induced HPMVEC injury.But the role of PHC up-regulating β-arrestin-1 expression has no necessary connection with M3 receptor.
