Effect of Dll4-Notch signaling pathway blockade on development of Th17 cells in asthmatic mice
10.3969/j.issn.1000-4718.2017.05.016
- VernacularTitle:阻断Dll4-Notch信号通路对哮喘小鼠Th17细胞分化的影响
- Author:
Weixi ZHANG
;
Cuiye WENG
;
Xiaoxiao JIA
;
Tingting ZHU
;
Zeyu ZENG
;
Ludan KONG
;
Lingzhi PAN
- Keywords:
Asthma;
Delta-like ligand 4;
Notch receptor;
T helper 17 cells;
Interleukin-17
- From:
Chinese Journal of Pathophysiology
2017;33(5):865-870
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the effect of Delta-like ligand 4 (Dll4)-Notch signaling pathway blockade on the development of Thelper 17(Th17) cells in the asthmatic mice.METHODS:Male BALB/c mice were randomly divided into 5 groups:control group, asthma group, normal saline group, anti-Dll4 antibody group, and immunoglobulin G group.The protein expression of Dll4 was detected by immunohistochemical staining.The proportion of Th17 cells in mouse spleen isolated CD4+ T cells was measured by flow cytometry.The protein expression of Th17 transcription factor retinoid-related orphan receptor γt (RORγt) was determined by Western blot.The serum level of interleukin (IL)-17 was measured by enzyme-linked immunosorbent assay (ELISA).RESULTS:The expression of Dll4 in the lung tissues from asthma group significantly increased as compared with anti-Dll4 antibody group.The proportion of Th17 cells in CD4+ T cells was significantly down-regulated, and the protein expression of RORγt in the lung tissues was significantly reduced in anti-Dll4 antibody group compared with asthma group (P<0.05).Moreover, the serum level of IL-17 in anti-Dll4 antibody group was significantly reduced compared with asthma group (P<0.01).CONCLUSION:The blockade of Dll4-Notch signaling pathway inhibits the differentiation of Th17 cells in asthmatic mice.
