Correlation of IL-19 and its receptors with tissue remodeling in chronic rhinosinusitis
10.3969/j.issn.1000-4718.2017.05.025
- VernacularTitle:IL-19及其受体与慢性鼻-鼻窦炎组织重塑的相关性研究
- Author:
Xia LI
;
Lihong CHANG
;
Zizhen HUANG
;
Zhiyuan WANG
;
Xianzhen CHEN
;
Jun WANG
;
Gehua ZHANG
- Keywords:
Chronic rhinosinusitis;
Tissue remodeling;
Interleukin-19;
Matrix metalloproteinase-9;
Tissue inhibitor of metalloproteinase-1
- From:
Chinese Journal of Pathophysiology
2017;33(5):919-924
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the differential expression of interleukin-19 (IL-19) and its receptors (IL-20R1/IL-20R2) in the samples of chronic rhinosinusitis (CRS) with or without nasal polyps (CRSwNP and CRSsNP), and to investigate the correlation of IL-19 and its receptors with tissue remodeling factors, including matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitor of metalloproteinase (TIMP)-1 in CRS.METHODS:The polyps from CRSwNP patients (n=30), the sinus mucosa from CRSsNP patients (n=15) and the inferior turbinal mucosa of nasal septum de-viation patients (n=15) were collected.The expression of IL-19 and its receptors (IL-20R1/IL-20R2) in each group was detected by real-time RT-PCR and immunohistochemistry.The expression of tissue remodeling factors MMP-2, MMP-9 and TIMP-1 in different groups was detected by real-time RT-PCR and ELISA.RESULTS:Increased mRNA and protein expression levels of IL-19, IL-20R1, IL-20R2 and MMP-9 were observed in CRSwNP group compared with CRSsNP and control group (P<0.05), while elevated expression level of TIMP-1 was observed in CRSsNP group compared with CRSwNP and control group (P<0.05).The relative expression of MMP-2 among the 3 groups showed no obvious difference.The expression of IL-19 and its receptors showed significantly positive correlations with MMP-9 in CRSwNP (P<0.05).No significant correlation between IL-19 and/or its receptors with TIMP-1 in CRSwNP group was observed.CONCLUSION:The high expression levels of IL-19, IL-20R1 and IL-20R2 and their positive correlations with MMP-9 in CRSwNP indica-te that IL-19 and its receptors may be involved in the tissue remodeling of chronic rhinosinusitis.
