Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Hair-cell Like Cells in Vitro
10.3969/j.issn.1006-7299.2017.03.015
- VernacularTitle:体外诱导人脐带间充质干细胞向内耳毛细胞样细胞分化的研究
- Author:
Ting ZHANG
;
Fanglei YE
;
Fangxia GUAN
;
Shanshan MA
;
Xiaodan ZHU
;
Kun ZHAO
;
Le WANG
;
Hongmin LI
;
Shaojuan HAO
- Keywords:
hUC-MSCs;
Nneural stem cells;
Hair-cell like cells in the inner ear;
Neural differentiation
- From:
Journal of Audiology and Speech Pathology
2017;25(3):280-284
- CountryChina
- Language:Chinese
-
Abstract:
Objective To induce human umbilical cord mesenchymal stem cells (hUC-MSCs) to hair-cell like cells in the inner ear, using a two-step neural differentiation method.Methods The hUC-MSCs were obtained from human umbilical cords by tissue adherence culture,whose surface antigen CD29, CD34, CD44, CD45, CD90, HLA-ABC, and HLA-DR could be identified by flow cytometry.In the neural stem cells induced phase, the NSE positive cells were analyzed by microscope and immunohistochemistry.In the second stage, the expression of hair-cell like cells markers (Math1, MyosinⅦa, Brn3c) were tested by qRT-PCR and immunofluorescence method.Results The control group and the protocol group had little NSE after differentiation while the protocol B group presented a neurobiological structure and demonstrated a higher NSE positive ratio after 5 days' neural stem cells induction (P<0.05).Compared to the control group, the mRNA and protein level of Math1, MyosinⅦa, and Brn3c exhibited a significant increase in the differential group,which induced for 4 weeks in the hair-cell like cells in the inner ear's induced phase(P<0.05).Conclusion The two-stage induction (hUC-MSCs-neural stem cells-hair-cell like cells) could produce more MyosinⅦa,Brn3c and Math1,which may provide an appropriate way to treat sensorineural deafness.
