Evaluation of neutralizing antibodies in serum immunized with virus-like particle chimerized HCV series of neutralizing epitopes
10.3969/j.issn.1000-484X.2017.05.015
- VernacularTitle:HCV多中和抗原表位嵌合病毒样颗粒免疫获得血清内中和抗体评价
- Author:
Xiaoyan WANG
;
Hai ZHANG
;
Yingfeng LEI
;
Fang LIN
;
Ying CUI
;
Bin LI
;
Huizhong ZHANG
;
Sanhua WEI
- Keywords:
Hepatitis C virus(HCV);
Epitope;
Virus-like particle;
Neutralizing antibody;
Immunization
- From:
Chinese Journal of Immunology
2017;33(5):707-711,720
- CountryChina
- Language:Chinese
-
Abstract:
Objective:New Zealand rabbits were immunized with VLPs-MEpS,VLPs-E2S,and the levels of neutralizing antibodies in serum were determined.Methods: New Zealand rabbits were immunized with 10 μg VLPs-MEpS and VLPs-E2S,serum was collected at diffferent time with a two-weeks interval.The neutralizing antibodies were determined by ELISA.HCV(type 1b) had been prepared and mixed with serum from immunized rabbit before infected Huh7.5 cell.The protection of neutralizing antibodies in serum was assessed.Results: Neutralizing antibodies had been induced in rabbit after immunized with VLPs-MEpS and VLPs-E2S.VLPs-MEpS group had higher titer of antibodies than that of VLPs-E2S group(P<0.05),both group had higher titer of antibodies than that of control groups significantly(P<0.01).VLPs-MEpS group had higher neutralization than that of VLPs-E2S group(P<0.05),the highest neutralization rate was 61.49%.Both groups were higher than control group notably(P<0.01).Conclusion: Protective neutralizing antibodies have been induced in New Zealand rabbit after immunized with VLPs-MEpS and VLPs-E2S.It′s the basement for development of neutralizing antibodies vaccine.
