With rapid latex scattering immune turbidimetric method to detect the urine retinol binding protein and evaluate methodology
10.3969/j.issn.1005-1678.2017.05.007
- VernacularTitle:快速乳胶散射免疫比浊法检测尿视黄醇结合蛋白方法学评价
- Author:
Yuedan JIN
;
Lin WANG
- Keywords:
latex scattering;
immune turbidimetry;
urine retinol binding protein;
methodology to evaluate
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(5):24-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective The rapid latex scattering immune turbidimetric method detecting urine retinol binding protein, and evaluate methodology.MethodsIn accordance with the commission of American clinical laboratory standards (NCCLS) requirements, evaluation of the research methods of reagent sensitivity, precision, accuracy, stability, linear range, interference analysis, specific degrees.ResultsWith rapid latex scattering immune turbidity method to detect the urine retinol binding protein, for instance, the minimum detection limit of 0.0381 mg/L;Repeatability precision is 1.198%, intermediate precision is 5.541%, reproducibility precision is 6.662%;The linear range is within 0~10 mg/L;Recovery rate were 99.00% and 104.00% respectively;When TBIL<100mg/L and Hb<10mg/L, the detection of Tbil, Hb, RBP interference;With the urine RBP than kit in automatic analyzer compared the test results of analysis showed that r2=0.9720, correlation can be;50 cases of clinical specimens using two methods showed no statistically significant difference positive rate (χ2=0.948, P=0.948).ConclusionRapid latex scattering immune turbidimetric method were used to detect the urine retinol binding protein, has high precision, high accuracy, the advantages of high sensitivity, stability and anti-jamming is strong, good.
