Repair effect of ultrasound microbubble on injuried facial nerve by bFGF transfection in rat
10.3969/j.issn.1671-8348.2017.13.006
- VernacularTitle:超声微泡对碱性成纤维生长因子转染大鼠损伤面神经的修复作用研究
- Author:
Junbo SU
;
Wenlong LUO
;
Yaning HAO
;
Deping WANG
- Keywords:
ultrasound mircobubble;
basic fibroblast growth factor;
facial nerve injury
- From:
Chongqing Medicine
2017;46(13):1747-1749
- CountryChina
- Language:Chinese
-
Abstract:
Objective To apply the ultrasound microbubble to mediate basic fibroblast growth factor(bFGF) for conducting the injuried facial nerve(rat model) repair and to investigate its feasibility and efficiency.Methods After establishing the models of facial nerve injury,40 SD rats were divided into 4 groups,10 cases in each group:group A,bFGF +ultrasound+microbubble(bFGF + MB/US),group B,bFGF and microbuble(bFGF+ MB),group C,bFGF and ultrasound(bFGF + US) and group D,simple operation(PBS).The general status of rats on 1,10,20,28 d after bFGF gene transfection was observed.The nerve conduction velocity (NCV),incubation period and amplitude of facial nerve action potential were measured.After taking the facial nerve tissue in injuried site,mRNA expression was detected by RT-PCR.Western blot was used to detect the bFGF protein expression.Results On 20 d after transfection,small swing of a small quantity of beard in the operation site of the group A could be observed;on 28 d after transfection,the general slatws of recavely in rats in the group A was better than that in the group B,C and D.The nerve electrophysiology manifestations after facial nerve repair in the group A were superior to the group B,C and D;the amount of bFGF mRNA and protein pxpression in the group A was significantly higher than that in the group B,C and D.Conclusion Ultrasound microbubble mediated bFGF is conducive to the repair of facial nerve injury.
