Effect of intestinal fatty acid binding protein gene G54A polymorphism on lipid metabolism in obesity patients
10.3760/cma.j.issn.1008-6315.2017.05.001
- VernacularTitle:小肠型脂肪酸结合蛋白基因G54A单核苷酸多态性对肥胖患者脂代谢的影响
- Author:
Zhenhui WANG
;
Guili ZHANG
;
Xiaotong CHANG
- Keywords:
Obesity;
Intestinal fatty acid-binding protein;
Gene polymorphism
- From:
Clinical Medicine of China
2017;33(5):385-389
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between intestinal fatty acid binding protein(FABP2) gene G54A polymorphism and obesity,the effect of mutant 54A FABP2 gene on serum lipids metabolism.Methods The total of 84 subjects with obesity and 60 subjects with normal weight were involved in this study.The G54A FABP2 gene allele and genotype frequencies were detected by polymerase chain reaction (PCR)-restriction fragment length polymorphism(RFLP) technology.The automatic biochemical Analyzer was used to detect triglyceride(TG),high-density lipoprotein cholesterol(HDL-C) and low-density lipoprotein cholesterol(LDL-C) levels.Results The results of study on FABP2 gene polymorphism revealed as followed:in obese groups,the frequencies of GG,GA,A/A genotypes was 19.0%(16/84),73.8%(62/84) and 7.2%(6/84),respectively;in control group,the frequencies of G/G,G/A,A/A genotypes was 38.3%(23/60),58.3%(35/60),3.3%(2/60),respectively;the differences between two groups was statistically significant(χ2=6.97,P<0.05).In obesity group,the frequencies of alleles were 56.0%(94/168) for 54G and 44.0%(74/168) for 54A;in the control group,the frequencies of alleles were 67.5%(81/120) for 54G and 32.5%(39/120) for 54A;the differences between two groups was statistically significant(χ2=3.92,P<0.05).The plasma biochemical variables results showed as followed:in obesity group,the carriers of A/A homozygous genotypes and G/A heterozyous genotypes both had significantly higher plasma TG((2.36±0.82) mmol/L,(2.06±0.59) mmol/L,respectively)and LDL-C((3.94±0.96) mmol/L,(3.29±0.55) mmol/L,respectively)level than those with GG wild genotype(t=2.206,2.575,2.632,2.278;P<0.05).The level of HDL-C in the carriers of A/A homozygous genotypes((1.23±0.34) mmol/L)and in the carriers of G/A heterozyous genotypes((1.21±0.26) mmol/L) were not significantly different than those with GG wild genotype((1.29±0.31) mmol/L,P>0.05).The carriers of A/A homozygous genotypes had significantly higher plasma LDL-C((3.94±0.96) mmol/L)level than thosewith G/A wild genotype((3.94±0.96) mmol/L vs.(3.29±0.55) mmol/L,t=2.476,P<0.05),but the plasma TG((2.36±0.82) mmol/L vs.(2.06±0.59) mmol/L;P>0.05) and HDL-C((1.23±0.34) mmol/L vs.(1.21±0.26) mmol/L;P>0.05) level had not difference.Conclusion The FABP2 gene G54A polymorphism is related to obesity and lipid metabolism abnormality.The allele encoding in FABP2 gene may be a potential factor contributing to promoting lipid metabolism abnormality.
