Curcumin reverses hepatocyte growth factor-induced resistance to gefitinib in PC9 lung cancer cells
10.3969/j.issn.1000-4718.2017.05.007
- VernacularTitle:姜黄素逆转HGF诱导PC9细胞对吉非替尼的耐药
- Author:
Jianwei ZHAN
;
Jian WANG
;
Yi WANG
;
Deming JIAO
;
Jun CHEN
;
You LI
;
Jinhong WU
;
Qingyong CHEN
- Keywords:
Curcumin;
Hepatocyte growth factor;
Gefitinib;
Drug resistance;
Epithelial-mesenchymal transition
- From:
Chinese Journal of Pathophysiology
2017;33(5):805-810
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the molecular mechanism through which curcumin reverses hepatocyte growth factor (HGF)-induced resistance to gefitinib in lung cancer cells.METHODS:The methods of MTT assay, wound healing assay and Western blot were used to observe the effects of HGF, curcumin and gefitinib on the migration, drug susceptibility, epithelial-mesenchymal transition, and related signaling pathways in the PC9 lung cancer cells.RESULTS:HGF reduced susceptibility of the PC9 cells to gefitinib, and curcumin significantly reversed HGF-induced resistance to gefitinib.HGF induced migration and epihelial-mesenchymal transition, and promoted c-Met/AKT/mTOR pathway activation in the PC9 cells.Gefitinib alone did not prevent the above activities.However, combined with curcumin, gefitinib prevented the above activities.CONCLUSION:Curcumin reverses HGF-induced resistance of the PC9 cells to gefitinib by preventing epithelial-mesenchymal transition and inhibiting c-Met/AKT/mTOR activation.
