Transplantation of peripheral purified CD34+ cells in treatment of thromboangiitis obliterans
10.3760/cma.j.issn.1007-631X.2017.04.012
- VernacularTitle:纯化CD34+细胞移植治疗血栓闭塞性脉管炎
- Author:
Zhihui DONG
;
Zheng WEI
;
Weiguo FU
;
Yuan FANG
;
Tianyue PAN
;
Bin CHEN
;
Daqiao GUO
;
Xin XU
;
Peng LIU
;
Junhao JIANG
;
Jue YANG
;
Zhenyu SHI
;
Ting ZHU
;
Yun SHI
- Keywords:
Thromboangiitis obliterans;
Cell transplantation;
Critical limb ischemia;
CD34+
- From:
Chinese Journal of General Surgery
2017;32(4):323-327
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the safety and efficacy of transplantation of purified CD34 + cells (PCCs) in treatment of critical limb ischemia (CLI) caused by thromboangiitis obliterans (TAO).Methods From May 2009 to June 2015,34 TAO-induced-CLI cases underwent PCCs transplantation.None of these patients were eligible for surgical or endovascular revascularization.G-CSF was subcutaneously injected for 5 days before peripheral CD34 + cells were isolated,purified and intramuscularly injected in the limbs.Patients were regularly follow-up.Results Technical success was achieved in all cases.The mean number of transplanted cells was (7.5 ± 2.4) × 105/kg.The follow-up was accomplished in 32 cases,ranging from 6 to 79 months (mean 45 ±24 months),and two patients were lost.Wong-Baker FACES pain rating scale score significantly decreased from 8.0 ±2.0(4-10)to 2.2 ±3.1 (P ＜0.05) at 1 month.The Peak pain-free walking time improved from (4.0 ± 2.0) min to (13.5 ± 5.3) min (P ＜ 0.05) at 3 months and (19.0 ± 3.1) min (P ＜ 0.05) at 6 months.The ankle-brachial index increased from 0.42 ± 0.20 to 0.50 ± 0.10 (P ＜ 0.05) at 3 months and 0.52 ± 0.11 (P ＜ 0.05) at 6 months,respectively.Transcutaneous partial oxygen pressure rose from (25 ± 11) mmHg to (48 ± 11) mmHg(P ＜ 0.05) at 3 months and (58 ± 10) mmHg (P ＜ 0.001) at 6 months,respectively.Ulcers healed in 21 out of 22 patients at (5 ± 4) months.The overall amputation-free survival rate was 94.1％ at 6 months and 91.2％ at 48 months.No major adverse events were observed perioperatively or postoperatively.Conclusions Transplantation of PCCs could yield safe,satisfactory and durable treatment outcomes in patients with TAO-induced-CLI.
