Model of neuroinflammation associated with cognitive dysfunction established by intraperitoneal injection of LPS
10.3969/j.issn.1000-4718.2017.05.020
- VernacularTitle:腹腔注射LPS建立认知功能障碍相关的中枢神经系统免疫炎症小鼠模型
- Author:
Xiaofeng CHENG
;
Jiayi ZHAO
;
Yu YUAN
;
Xin LAN
;
Qiongru YUAN
;
Cuiqin HUANG
;
Daxiang LU
;
Lihong ZHU
;
Wei BI
- Keywords:
Cognitive dysfunction;
Lipopolysaccharide;
Neuroinflammation
- From:
Chinese Journal of Pathophysiology
2017;33(5):890-895
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish a mouse model of immuno-inflammation in central nervous system (CNS) associated with cognitive dysfunction.METHODS:C57BL/6J male mice were divided into 3 groups.Lipopolysaccharide (LPS) was intraperitoneally injected into the mice to induce cognitive impairment.Morris water maze test, passive avoidance test and pole test were used to observe the behavioral changes of mice.The histomorphology was analyzed by the method of immunofluorescence.The detailed molecular mechanism was determined by Western blot.RESULTS:Compared with saline group, LPS induced mouse sickness behavior and memory loss.Microglia activation and neuronal loss in the hippocampus were observed.The expression of neuroinflammatory proteins COX-2 and iNOS in the brain of LPS-induced mice was increased.CONCLUSION:Intraperitoneal injection of LPS induces cognitive dysfunction in mice.
