Effects of bcl-3 gene silencing on apoptosis of human colon cancer cell line RKO
10.3969/j.issn.1000-4718.2017.05.029
- VernacularTitle:bcl-3基因通过cyclin D1及Bax蛋白影响人结肠癌RKO细胞的凋亡
- Author:
Shaomin WANG
;
Meng YE
;
Shumin NI
;
Xiaolei YE
- Keywords:
bcl-3;
Colorectal neoplasms;
Apoptosis;
Cell migration
- From:
Chinese Journal of Pathophysiology
2017;33(5):939-943
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of bcl-3 gene on the migration and apoptosis of human colon cancer cell line RKO, and the changes of cyclin D1 and apoptosis-related proteins.METHODS:After silencing of bcl-3 gene expression in human colon cancer cell line RKO by lentiviral vector with RNA interference, the changes of RKO cell migration ability were investigated by wound healing assay.The changes of RKO cell apoptotic rate after bcl-3 gene silencing were detected by flow cytometry with Annexin V/PI staining.The protein expression of cyclin D1 and apoptosis-related proteins in the RKO cells after bcl-3 gene silencing was determined by Western blot.RESULTS:The wound healing rates of the RKO cells were 84.00% and 40.00% before and after bcl-3 gene silencing, respectively, with statistically significant difference (P<0.05).Annexin V/PI staining showed that the cell apoptotic rates were 12.89% and 59.67% before and after bcl-3 gene silencing, respectively, when RKO cells were treated with 5 μmol/L cisplatin for 24 h, with statistically significant difference (P<0.05).The expression of cyclin D1 decreased, while the expression of Bax increased after bcl-3 gene silencing (P<0.05).CONCLUSION:After bcl-3 gene silencing, the migration ability of RKO cells decreases, and the apoptotic rate increases, accompanying with the changes of cyclin D1 and Bax.bcl-3 gene can affect the apoptosis of RKO cells by changing the expression of cyclin D1 and Bax.
