Effect of protein tyrosine kinase 6 on human airway epithelial barrier dsyfunction induced by TNF-α
10.3969/j.issn.1000-484X.2017.05.003
- VernacularTitle:蛋白酪氨酸激酶6对TNF-α诱导的人气道上皮屏障功能减损的影响
- Author:
Qing XU
;
Lingxiu CHEN
;
Xiangdong ZHOU
- Keywords:
Protein tyrosine kinase 6;
Tumor necrosis factor-α;
Airway epithelial barrier;
c-Jun N-terminal kinase;
p38 mitogen-activated protein kinase
- From:
Chinese Journal of Immunology
2017;33(5):652-655
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of protein tyrosine kinase 6(PTK6) on TNF-α induced human airway epithelial barrier dysfunction and mechamism.Methods: After cultivating 16HBE cells in vitro,the recombined PTK6 and PTK6 siRNA was respectively transfected into the cells,with empty vector and scramble siRNA as control.The cells were incubated with exogenous TNF-α.Cell viability was detected by MTT assay.Cells TER and permeability were detected.ZO-1 and Occludin mRNA were analyzed by RT-PCR.PTK6,ZO-1,Occludin,p-ERK1/2,p-JNK1/2 and p-p38MAPK protein were assayed by Western blot.Results: TNF-α remarkably decreased ZO-1 and Occludin mRNA and protein and TER;increased cells permeability,as well as p-ERK1/2,p-JNK1/2 and p-p38 protein(P<0.05).Upregulation of PTK6 further decreased ZO-1 and Occludin mRNA and protein and TER,enhanced cells permeability and p-JNK1/2 and p-p38 protein(P<0.05),and downregulated PTK6 ,the changes of these indices were opposite(P<0.05).Whereas upregulation or downregulation of PTK6 had no effect on p-ERK1/2.Conclusion: Downregulation of PTK6 inhibits the phosphorylation of JNK1/2 and p38MAPK,thus improving TNF-α-induced human airway epithelial barrier dysfunction.
