Effect of down-regulation of CD59 gene on apoptosis of acute T lymphocyte Jurkat cell lines
10.3969/j.issn.1000-484X.2017.05.012
- VernacularTitle:下调CD59对急性T系白血病细胞株Jurkat 凋亡相关分子的影响
- Author:
Meihua GAO
;
Huaqiao LI
;
Bing LI
;
Zhong WANG
;
Beibei CONG
;
Bing WANG
- Keywords:
CD59;
Apoptosis;
Jurkat;
Transfection
- From:
Chinese Journal of Immunology
2017;33(5):693-696
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the change of related molecular about apoptosis we reduce the expression of CD59 on acute T lymphocyte Jurkat cell lines .Methods: RNA interference (RNAi) was used to reduce the expression of CD59 gene by lentivirus,confocal was applyed to observe the transfection efficiency and the location of CD59 molecular then Real-time-PCR and Western blot were used to select the most effective group to do the rest experiment;Western blot was used to detect the change of expression about Bcl-2,Bax,Caspase-3 and Survivin;ELISA was used to investigate the expression of IL-3 and TNF-β.Results: Confocal observed each group′s transfection efficiency over 90%,CD59 molecules were mainly located in cell membrane;Real-time-PCR and Western blot showed group A had the best down-regulation efficiency;we defined RNAi-CD59-A as experimental group for subsequent experiments named RNAi-CD59;the experimental group can enhance the expression of Bax,caspase-3 (P<0.05),inhibit the expression of Bcl-2 and Survivin(P<0.05);ELISA showed that the expression of IL-3 in the down-regulation group increase(P<0.05),the expression of TNF-β decrease (P<0.05).Conclusion: Down-regulation CD59 can promote the expression of apoptosis molecular in acute leukemia Jurkat cell lines restrain the expression of proliferation molecular.
