Association of microsatellite polymorphism of MICA gene with susceptibility to esophageal cancer
10.3969/j.issn.1000-484X.2017.05.021
- VernacularTitle:MICA 第5外显子微卫星多态性与食管癌相关性研究
- Author:
Qingfeng ZHENG
;
Zhifeng ZHOU
;
Shuoyan LIU
;
Wansong LIN
;
Saiyun CHEN
;
Yunbin YE
- Keywords:
MICA gene;
Microsatellite polymorphism;
Esophageal;
NK cells
- From:
Chinese Journal of Immunology
2017;33(5):738-741,745
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the association of microsatellite polymorphism of MICA gene with susceptibility to esophageal cancer.Methods: PCR-STR microsatellite genotyped technique was used to detect the polymorphism of MICA in Exon 5 in 103 cases of esophageal cancer and 84 cases of normal controls.Constructed of eukaryotic expression vector in esophageal carcinoma with high frequency of occurrence of the MICA allele.NK cells killing effect to 293T cells after alleles MICA transfected were assayed by LDH and the effect on target was 20∶1.ELISA was used to test supernatants sMICA of 293T cell after transfected.Results: Identified five allelic genes in MICA Exon 5 with esophageal cancer.Each allele and its frequency respectively were:MICA-A4(9.71%),MICA-A5(22.3%),MICA-A5.1(40.8%),MICA-A6(15.5%),MICA-A9(11.7%).MICA-A5.1 showed significant difference comparison with the control group.After 293T cell line was transfected MICA allele,MICA-A5.1 group was less sensitive to NK cytotoxicity compared to other groups[(30.4±6.3)%,P<0.05].The secretion of soluble MICA increased(135.7±6.2)pg/ml.Conclusion: Esophageal cancer was relevent with the MICA-A5.1 polymorphism of MICA Exon 5 alleles.Its risk is higher than other alleles.
