The experimental research of Neutrophil Gelatinase-associated Lipocalin(NGAL) siRNA encapsulated by UAC on NGAL gene silence in colon cancer cells
10.3969/j.issn.1005-1678.2017.05.004
- VernacularTitle:尿刊酸修饰纳米壳聚糖载体负载NGAL siRNA干扰结肠癌细胞NGAL基因的实验研究
- Author:
Xiufeng ZHANG
;
Houdong WANG
;
Zhong SHEN
;
Guangen YANG
;
Jieli PAN
;
Meiya LI
- Keywords:
colon cancer;
NGAL;
chitosan;
RNA interference
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(5):14-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of NGAL knockdown by NGAL siRNA encapsulated with urocanic acid-modified chitosan nanoparticles (UAC).MethodsNGAL siRNA encapsulated by UAC and chitosan (CTS) respectively, which were then used to transfect human colon cancer cell lines HT29.The expression level of NGAL protein were detected by Enzyme Linked Immunosorbent Assay(ELISA).ResultsThe ELISA study revealed that the expression level of NGAL protein in UAC group(average 0.583μg/L) was significantly lower than in CTS group (average 0.772μg/L) and control group(average 1.071μg/L) (P<0.05).ConclusionThe NGAL expression of mRNA and protein in HT29 cells could be down-regulated by siRNA encapsulated by UAC.
