Effects of exogenous interleukin-10 on STAT3 protein activation in rats with allergic rhinitis
10.3969/j.issn.1005-1678.2017.05.005
- VernacularTitle:外源性白细胞介素-10对变应性鼻炎大鼠STAT3蛋白活化的影响
- Author:
Xiaoming SU
;
Guoli LI
;
Aiyan JIN
;
Baoqiang DAI
;
Bin WANG
;
Weiwei LIU
- Keywords:
allergic rhinitis;
IL-10;
STAT3
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(5):18-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of exogenous interleukin-10 (IL-10) on STAT3 protein activation in rats with allergic rhinitis (AR).Methods60 SD rats were randomLy divided into AR model group, IL-10 group and control group, with 20 in each group.AR group and IL-10 group were treated with conventional ovalbumin (OVA) combined with aluminum hydroxide sensitization method.The negative control group was treated with saline instead of 48 h after the last challenge.The levels of IL-6, TGF-β and P-STAT3 in nasal mucosa and serum IgE were measured by ELISA kit.The expression of IL-6, TGF-β and P-STAT3 in nasal mucosa was detected by Western-blot Detection.ResultsAR group exhibited severe clinical manifestations of AR, such as friction around, tears flowing, the signs of which in IL-10 group were significantly lower than that in the control group (P<0.05).Compared with the control group, the levels of serum IgE and IL-6, TGF-β and P-STAT3 in the nasal mucosa were significantly higher than those in the control group (P<0.05).After treatment with IL-10, the levels of TGF-β and P-STAT3 were significantly increased (P<0.05).Western-blot showed that compared with the control group IL-6, TGF-β and P-STAT3 in nasal tissues of AR group and IL-10 group were significantly increased, while the expression level of IL-10 group was lower than that of AR group.ConclusionTreatment of AR with exogenous IL-10 can significantly relieve the clinical manifestation of AR, inhibit STAT3 activation immune response, and achieve the purpose of treatment of AR in some extent.
