Determination of glycerol in propofol medium and long chain fat emulsion injection by HPLC

VernacularTitle:高效液相色谱法测定丙泊酚中/长链脂肪乳注射液中甘油的含量
Author: Chaodong ZHOU ; Zhe SU ; Bing MA ; Haijiao BAI ; Zhesu HUANG
Keywords: high performance liquid chromatographic (HPLC); propofol medium and long chain fat emulsion injection; glycerol; content determination
From: Chinese Journal of Biochemical Pharmaceutics 2017;37(5):21-23
CountryChina
Language:Chinese
Abstract: Objective A high performance liquid chromatographic (HPLC) method was established for the determination of glycerol in propofol medium and long chain fat emulsion injection.MethodsThe chromatographic conditions were as follows: Kromasil 100-5-NH2 column(4.6×250mm,5μm) with the column temperature was 40℃,acetonitrile-water(8515)as mobile phase with flow rate of 1.0mL/min.Glycerol was detected by refractive index (RI) detector at 40℃.ResultsThe linear range of glycerol was 455.3916-2276.9580μg/mL(r=0.9999,n=7),the average recovery rate was 99.5%,RSD was 0.6%(n=9),the limit of detection(LOD) was 121ng and the limit of quantification(LOQ)was 364ng.ConclusionThe method was simple, rapid, strong specifity and accurate with good reproducibility, which is suitable for the content determination of glycerol in propofol medium and long chain fat emulsion injection.