Effect of low dose clomiphene in treatment of idiopathic oligoasthenospermia and therapeutic effect on sperm epididymal protein 4 levels
10.3969/j.issn.1005-1678.2017.05.016
- VernacularTitle:低剂量克罗米芬治疗特发性少精弱精症的效果及对精浆附睾蛋白4水平的影响
- Author:
Fei SHI
;
Huirong YIN
;
Di SONG
;
Shasha LIN
;
Yajun XU
- Keywords:
idiopathic oligospermia and asthenospermia;
clomiphene citrate;
vitamin E;
epididymis protein 4;
oxidative stress
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(5):54-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate therapeutic effect of the low dose clomiphene treatment of idiopathic oligoasthenospermia and effect on sperm epididymal protein level 4.Methods183 cases of idiopathic oligoasthenospermia were divided into three groups, vitamin group of 61 patients were given vitamin E,clomiphene group(61 cases)treated with low dose clomiphene, combined group of 61 patients were given low dose clomiphene and vitamin E, lasted for 12 weeks.Semen volume, sperm density, sperm motility, sperm survival rate and sperm reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), human epididymis protein 4 (HE4), follicle stimulating hormone, luteinizing hormone, testosterone concentration were observed before and after treatment.After treatment, the patients were followed up for 1 year, the observation of the natural pregnancy rate after treatment.ResultsSperm density, sperm survival rate and sperm and a A+B grade sperm in the clomiphene group were significantly higher comparedwith the vitamin group (P< 0.05), sperm density, sperm survival rate and sperm and a A+B grade sperm in the combined group were significantly increasedcompared with clomiphene group and vitamin group (P< 0.05),levels of HE4 after treatment was significantly lower compared with before treatment (P< 0.05), And the difference between the three groups were statistically significant (P< 0.05).Follicle stimulating hormone, luteinizing hormone, testosterone in clomiphene group and combined group after treatment, were significantly increased compared with before treatment (P< 0.05), and the combined group had no statistically difference compared with clomiphene group.The pregnancy rate in 1 year of vitamin group, clomiphene group and combined group were 24.59%, 42.62% and 62.29% (P< 0.05).ConclusionClomiphene treatment of idiopathic asthenospermia is better than vitamin E, the combination of the two has a synergistic effect.
