Effect of curcumin on radiosensitization of radioresistantnasopharyngeal carcinoma cell line CNE-2R and its mechanism
10.3969/j.issn.1001-1978.2017.08.010
- VernacularTitle:姜黄素对鼻咽癌抗拒株的放射增敏作用及其机制研究
- Author:
Daoqi ZHU
;
Mu HUANG
;
Zhaoru LIU
;
Aiwu LI
;
Meng SHAO
;
Yuanliang LIU
;
Miao FANG
;
Jiabin YANG
;
Ying LYU
;
Zhixian MO
;
Qin FAN
- Keywords:
curcumin;
nasopharyngeal carcinoma;
radiosensitization;
colony formation assay;
cell cycle;
RT-qPCR
- From:
Chinese Pharmacological Bulletin
2017;33(8):1086-1091
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of curcumin on radiosensitivity of radioresistant nasopharyngeal carcinoma cell line CNE-2R and its mechanism.Methods The concentration of curcumin was screened by MTT assay.Dose-survival curves were obtained according to the colony forming test for L-Q matching and multitarget-single hitting matching,while SF2 and the correlation parameters of radiation biology were calculated.The changes of cell cycle in CNE-2R cells caused by curcumin were also tested by flow cytometry(FCM).The differential expression of genes related to cell cycle and DNA damage repair were detected by RT-qPCR.Results CNE-2R cells could not be inhibited by 10 μmol·L-1 curcumin.Dealt with 10 μmol·L-1 curcumin for 24 h,the value of α/β increased to 1 596 from 6.56;the value of SF2 decreased to 0.361 Gy from 1.93 Gy;the value of N decreased to 1.06 from 1.60;the value of D0 decreased to 2.12 from 3.27;the value of Dq decreased to 0.12 from 1.53.FCM showed that the cells in G2 phase had a significant increase and the cells in S phase had a significant decrease after dealt with 10 μmol·L-1 curcumin for 24 h.The expression of CDK4 was significantly up-regulated and GADD45g,BRCA1 were significantly down-regulated.Conclusion Curcumin radiosensitizes nasopharyngeal carcinoma cell line CNE-2R by changing cell cycle and affecting DNA damage repair through regulating the expression of CDK4,GADD45 g and BRCA1.
