Calcium antagonists protect cardiac microvascular endothelialcells against hypoxia/reoxygenation injury through iPLA2
10.3969/j.issn.1001-1978.2017.08.016
- VernacularTitle:钙拮抗剂调节钙非依赖磷脂酶A2拮抗心脏微血管内皮细胞缺氧/复氧损伤的研究
- Author:
Qiaoling ZHOU
;
Yuanhang WANG
;
Hong LIN
;
Bin WANG
;
Ganggang SHI
;
Fuchun ZHENG
- Keywords:
calcium antagonist;
N-n-butyl haloperidol iodide;
Ca2+-independent phospholipase A2;
arachidonic acid;
cardiac microvascular endothelial cells;
hypoxia/reoxygenation
- From:
Chinese Pharmacological Bulletin
2017;33(8):1119-1125
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of classic calcium antagonists verapamil(Ver),nifedipine(Nif),diltiazem(Dil)and the novel calcium antagonist N-n-butyl haloperidol iodide(F2)which was synthesized by our lab by regulating Ca2+-independent phospholipase A2(iPLA2)on hypoxia/reoxygenation(H/R)injury of cardiac microvascular endothelial cells(CMECs)and the mechanisms.Methods The CMECs were isolated from SD neonatal rats.The H/R model was established,then cells were treated with different concentrations of calcium antagonists and F2.The content of LDH in the cell supernatant was measured by colorimetric method.The levels of IL-6 and AA in cell supernatant were measured by ELISA;and late-stage apoptosis was measured by TUNEL.The mRNA and protein expression levels of iPLA2 in CMECs were examined by real time-PCR and Western blot analysis.Results Calcium antagonists except Dil decreased the generation of LDH,IL-6 and AA in a dose-dependent manner(P<0.05),and reduced the apoptosis(P<0.05).F2 and Ver decreased the mRNA and protein expression of iPLA2 in a dose-dependent manner,while there were no such effects for Nif and Dil.Conclusions Calcium antagonists except Dil have protective effects against H/R injury.F2 and Ver protect CMECs against H/R injury partly through iPLA2.
