Effects of Hawthorn Leaf Procyanidins on Over Expressions of ICAM-1 and E-selectin in Human Umbilical Vein Endothelial Cells Induced by TNF-α
10.3969/j.issn.1005-5304.2017.08.012
- VernacularTitle:山楂叶原花青素对肿瘤坏死因子-α诱导人脐静脉内皮细胞细胞间黏附分子-1和E-选择素过表达的影响
- Author:
Peng LI
;
Jiannong WANG
;
Yonggang XU
;
Jianhua FU
;
Jianxun LIU
- Keywords:
hawthorn leaf;
procyanidins;
vascular endothelial cell;
TNF-α;
ICAM-1;
E-selectin
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(8):50-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of hawthorn leaf procyanidins (HLP) on over expressions of ICAM-1 and E-selectin in human umbilical vein endothelial cells (HUVEC) induced by TNF-α,and clarify the mechanism of HLP's anti-inflammation effect. Methods HUVEC were cultured in vitro. MTT assay was used to detect cell viabilities. The expressions of ICAM-1 and E-selectin in HUVEC were detected by flowcytometry. Results Up to 200 mg/L, HLP showed no significant decrease in cell viabilities; the expression levels of ICAM-1 and E-selectin in the model group significantly increased, compared with that in the normal group; 10, 20, 30, 40, 50 mg/L HLP inhibited the expression elevations of ICAM-1 and E-selectin in concentration-dependent manner; and there were statistical significances in 40, 50 mg/L HLP groups, compared with the model group. Conclusion HLP can inhibit the over expressions of ICAM-1 and E-selectin of vascular endothelial cells induced by TNF-α, which possibly underlies HLP's anti-inflammation effect.
