Content Determination of Four Flavonoids in Hedysari Radix in Gansu Province Based on Quantitative Analysis of Multi-components by Single Marker
10.3969/j.issn.1005-5304.2017.08.015
- VernacularTitle:基于一测多评法测定甘肃红芪中4种黄酮类成分
- Author:
Xiujuan YANG
;
Jing SHAO
;
Zhijun YANG
;
Guoxia WU
;
Yanmei NING
;
Jinbao ZHANG
;
Shengrui HEI
- Keywords:
quantitative analysis of multi-components by single marker;
Hedysari Radix;
ononin;
calycosin;
genistein;
formononetin;
relative correction factor
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(8):66-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for simultaneous determination for the contents of four flavones (ononin, calycosin, genistein and formononetin) of Hedysari Radix in Gansu Province with quantitative analysis of multi-components by single-marker (QAMS); To prove its feasibility and accuracy. Methods Calycosin was taken as internal standard substance. Relative correction factors (RCF) of ononin, genistein and formononetin to calycosin were established. The contents of ononin, calycosin, genistein and formononetin were determined to realize QAMS. Results RCF was with good repeatability. The results of QAMS were consistent with the results of the external standard method. Conclusion The method that determines the contents of ononin, genistein and formononetin with calycosin as internal standard substance, can be used for quantitative analysis of Hedysari Radix.
