Comparison of Effects of Different Drying Methods on Contents of Effective Components of Forsythiae Fructus Leaves Tea
10.3969/j.issn.1005-5304.2017.08.017
- VernacularTitle:不同干燥条件连翘叶茶有效成分含量比较
- Author:
Jingmei MA
;
Yingchun WANG
;
Chen LI
;
Meng WANG
;
Qian ZHENG
;
Liying NIU
- Keywords:
Forsythiae Fructus leaves tea;
total flavonoids;
forsythoside A;
phillyrin;
UVspectrophotometry;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(8):76-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the contents of total flavonoids, forsythoside A and phillyrin in Forsythiae Fructus leaves tea under different drying conditions; To determine the best drying method for Forsythiae Fructus leaves tea. Methods With the sodium nitrite-aluminum trichloride-sodium hydroxide solution as color reagent, total flavonoids in forsythiae Fructus leaves tea were determined by UV spectrophotometry at the wavelength of 500 nm. HPLC was used to determine the contents of forsythiaside A and phillyrin. The analysis was performed on a Diamosil C18 (2) column (4.6 mm × 250 mm, 5 μm); the mobile phase was composed of acetonitrile and 0.4% acetic acid with gradient elution; the detection wavelength was set at 277 nm; the flow rate was 1.0 mL/min at column temperature of 30 ℃. Results The content of total flavonoids of Forsythiae Fructus leaves tea under different drying conditions was basically the same; the sequence of the contents of forsythoside A and Forsythin of Forsythiae Fructus leaves tea under different drying conditions was: ventilated drying > vacuum drying > blast drying. Conclusion Different drying conditions have no effect on the contents of total flavonoids in Forsythiae Fructus leaves tea, but have obvious effects on the contents of forsythiaside A and phillyrin. Ventilation shade is better than blast drying and vacuum drying for preverence of forsythiaside A and forsythin.
