Study on Species Homology and Quality Control of Two Kinds of Antrodia Cinnamomea
10.3969/j.issn.1005-5304.2017.08.019
- VernacularTitle:牛樟芝和沉水樟芝的物种同源性及质量控制研究
- Author:
Hongyun WANG
;
Gong WANG
;
Lin YU
;
Kedan CHU
;
Wei XU
;
Huang LI
- Keywords:
Antrodia Cinnamomea;
fingerprint;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(8):85-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare and study species homology of two kinds of Antrodia Cinnamomea; To conduct the study on quality control. Methods HPLC fingerprint of two kinds of Antrodia Cinnamomea was applied. The HPLC fingerprints were determined on an Diamonsil C18 (2) column (250 mm × 4.6 mm, 5 μm) eluted with the mobile phase consisting of 1% formic acid solution and acetonitrile in gradient mode at a flow rate of 0.8 mL/min; the detection wavelength was set at 254 nm; the column temperature was 30 ℃. Results There were 9 common peaks in the HPLC fingerprints of Antrodia Cinnamomea. The similarities varied from 0.906 to 0.995 and from 0.956 to 0.998 in 11 batches and 10 batches of two kinds of Antrodia Cinnamomea, respectively. Conclusion The method is simple and with good reproducibility, which shows that the HPLC fingerprint and infrared spectrum share similarity for two kindsof Antrodia Cinnamomea.