Identification of Ilicis Rotundae Cortex and Its Adulterant Based on HPLC Fingerprint
10.3969/j.issn.1005-5304.2017.08.020
- VernacularTitle:基于HPLC指纹图谱对救必应与其伪品的鉴别研究
- Author:
Chun WANG
;
Wei WANG
;
Cui WU
;
Ning DU
;
Wen SUN
;
Hui LI
;
Zhimao CHAO
- Keywords:
Ilicis Rotundae Cortex (Jiubiying);
Ilex godajam (Colebr) Wall.;
HPLC;
fingerprint;
principal component analysis;
quality control
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(8):89-92
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish HPLC fingerprints for the identification of Ilicis Rotundae Cortex (Jiubiying), dried bark of Ilex rotunda Thunb., and its adulterant, dried bark of Ilex godajam (Colebr.) Wall.. Methods Some samples named Jiubiying were collected from some medicine markets and drugstores. The HPLC fingerprints were obtained to detect Jiubiying and Ilex godajam. The software Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine (2004A edition) was used to evaluate fingerprints. Results The fingerprints of Jiubiying and Ilex godajam were established. Methodological study met the technical requirements of fingerprints. There were 31 and 28 common peaks in Jiubiying and Ilex godajam, respectively. Jiubiying and Ilex godajam could be classified into two clusters by principal component analysis and hierarchical clustering analysis. Conclusion Jiubiying and Ilex godajam can be identified by HPLC fingerprints. This method can provide an effective method for quality evaluation of Jiubiying.