Screening, preparation and biological activity of human monoclonal antibodies against postsynaptic short-chain neurotoxins fromLapemis curtus
10.11855/j.issn.0577-7402.2017.07.07
- VernacularTitle:抗平颏海蛇短链神经毒素全人源单克隆抗体的筛选、制备及生物活性研究
- Author:
Shi HU
;
Yafeng SHEN
;
Tian LI
;
Changhai LEI
- Keywords:
Lapemis curtus;
short chain neurotoxin;
monoclonal antibodies;
fully human antibodies;
antitoxin
- From:
Medical Journal of Chinese People's Liberation Army
2017;42(7):612-616
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare human anti-postsynaptic neurotoxin monoclonal antibody from phage antibody library using recombined postsynaptic short-chain neurotoxins ofLapemis curtus.Methods The three postsynaptic neurotoxins were expressed inEscherichia coli and phage antibodies against neurotoxins were screened. The obtained scFvs were further constructed to full antibodies. The antigen binding ability, biochemical and pharmacokinetic characteristics of the depurated antibodies were evaluated, and the anti-toxin effects of the antibody drugs were verifiedin vivo.Results Two positive scFvs with specific binding ability to all the three neurotoxins were obtained after 4 rounds of panning, and then the full antibodies were generated, expressed and purified. Antibody binding specificity was further confirmed. Pharmacokinetics of these two antibodies, SM-SD-911 and SM-SD-861 were similar to a conventional IgG molecule. SM-SD-911 and SM-SD-861 also showed strong antitoxin effectin vivo. Conclusionfull human anti-postsynaptic neurotoxin antibody has been successfully obtained by using recombinant neurotoxin technology and a large phage antibody library which may achieve clinical efficacy in navy medical applications.
