Expression of TRPC 6 in renal tissue and its significance in children with primary nephrotic syndrome
10.3969/j.issn.1000-3606.2017.07.006
- VernacularTitle:儿童原发性肾病综合征肾组织瞬时受体电位阳离子通道蛋白6表达及意义
- Author:
Sheng HAO
;
Ying WU
;
Weixun HE
;
Yulin KANG
;
Wenyan HUANG
;
Guanghua ZHU
- Keywords:
nephrotic syndrome;
podocyte;
child
- From:
Journal of Clinical Pediatrics
2017;35(7):498-502
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the expression of transient receptor potential cation channel subfamily C member 6 (TRPC6) and podocyte injury in children with primary nephrotic syndrome (PNS) and its clinical significance. Methods The renal tissue of 18 children with PNS was obtained. The pathological changes of kidney were observed by routine section staining and light microscopy. The structural changes of podocyte were observed by electron microscope. The mRNA and protein expressions of TRPC6 in tissues were determined by qPCR and immunohistochemistry, respectively. Further the correlation of TRPC6 mRNA with serum levels of albumin (Alb), creatinine (Cr), triacylglycerol (TG), cholesterol (Tch), complement C3 and 24 h urinary protein quantitation and estimated glomerular filtration rate (eGFR) were analyzed respectively. Results The expression of TRPC6 protein in renal tissue of children with PNS was higher than that in the control group, and the difference was statistically different (P<0.05). The relative expression of TRPC6 mRNA in renal tissue of children with PNS was positively related to the expression of TRPC6 protein (r=0.508, P<0.05), but there was no correlation of expression of TRPC6 mRNA with serum levels of Alb, Cr, TG, Tch, C3, eGFR and 24h urinary protein quantitation (P>0.05). Conclusion The pathological types of PNS were mainly podocyte lesions, and the expression of TRPC6 protein was increased in podocytes. TRPC6 detection may be helpful in the diagnosis of podocyte lesions.
