Apoptosis of human hepatocellular carcinomaHepG-2 cells induced by total flavonoids of Verbena officinalis L through targeting topoisomerase Ⅱ
10.3969/j.issn.1001-1978.2017.08.021
- VernacularTitle:马鞭草总黄酮靶向拓扑异构酶Ⅱ诱导肝癌HepG-2细胞凋亡
- Author:
Xianjia LI
;
Liping REN
;
Shaoju JIN
- Keywords:
total flavonoids of Verbena officinalis L;
HepG-2;
cell apoptosis;
TopⅡ;
Bcl-2/Bax/caspase-3 signaling pathway
- From:
Chinese Pharmacological Bulletin
2017;33(8):1147-1152
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore whether total flavonoids of Verbena officinalis L(TFV)can induce apoptosis of HepG-2 cells through targeting topoisomerase Ⅱ.Methods HepG-2 cells were cultured with TFV at 200,100,50 mg·L-1.Cell apoptosis was evaluated by TUNEL-DAPI double staining.TopⅡ activity was detected by supercoiled pBR322 DNA relaxation assay.The levels of the mRNA of TOP Ⅱα,TOP Ⅱβ were analyzed by real time PCR.Expression of Bcl-2,Bax,TOP Ⅱα,TOP Ⅱβ and caspase-3 was analyzed by Western blot.
