Interacting of receptor component protein and G protein in static pressure-induced proliferation of VSMC
10.3969/j.issn.1001-1978.2017.08.025
- VernacularTitle:RCP与G蛋白偶联在静压力促血管平滑肌细胞增殖中的作用
- Author:
Xiaoyi ZHANG
;
Zhen WANG
;
Feng GUO
;
Yuhuan LIU
;
Li YANG
;
Fang YANG
;
Xuping QIN
- Keywords:
RCP;
static pressure;
vascular smooth muscle cells;
proliferation;
G protein;
signal transduction
- From:
Chinese Pharmacological Bulletin
2017;33(8):1170-1175
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of receptor component protein(RCP)in the signal transduction of vascular smooth muscle cell(VSMC)proliferation induced by static pressure.Methods The mouse-derived vascular smooth muscle cell line(A10VSMC)was employed in the experiment.Cells were exposed to static pressure,and MTT assay was used to detect the cell viability.Western blot was used to determine the expressions of PCNA,RCP and p-Akt,RCP mRNA was tested by RT-PCR,and co-immunoprecipitation was used to test the interaction between RCP and G proteins.Results The cell viability,expressions of PCNA and RCP increased with the elevation of static pressure and reached their peaks at 120 mmHg,and after 6 hours they got a plateau.The static pressure significantly increased the level of p-Akt,meanwhile,the binding of RCP and Gαs significantly decreased.However,the binding of RCP and Gβ increased in response to static pressure after stimuli of static pressure,but Gγ was obscure.Conclusion Static pressure can induce VSMC proliferation and expression of RCP,which may involve G protein signal transduction model.
