Safety evaluation of Ad-shRNA-NgR in brain tissue of EAE rats
10.11659/jjssx.01E017061
- VernacularTitle:Ad-shRNA-NgR转染EAE大鼠脑组织安全性评估
- Author:
Yan ZHANG
;
Xinyue QIN
;
Min ZHANG
;
Xi HE
;
Jiayu ZHANG
- Keywords:
adenovirus;
experimental autoimmune encephalomyelitis;
transfection;
safety;
animal model
- From:
Journal of Regional Anatomy and Operative Surgery
2017;26(7):483-487
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the survival of EAE rats after recombinant adenovirus NgR specific RNA interference(Ad-shRNA-NgR) transfected the brain tissue of the experimental autoimmune encephalomyelitis(EAE),and provide the basis for EAE intervention.Methods EAE rats were randomly divided into high,medium,low and control groups(20 rats in each group).The lateral ventricle of EAE rats were injected with a titer of 1×1011 pfu/mL,1×1010 pfu/mL and 1×109 pfu/mL Ad-shRNA-NgR.The survival of EAE rats at third and seventh days after injection was observed.Results The survival rate of EAE rats of the high titer group was significantly lower than those of the middle titer group and low titer group at third and seventh days after Ad-shRNA-NgR transfected into EAE brain tissue.There was no significant difference in survival rate in middle titer group,low titer group and control group.Conclusion The titer of Ad-shRNA-NgR is safe in the experiment of EAE rats from 1×1010 pfu/mL to the range of 1×109 pfu/mL.
