Effects of rhTIMP-3 combined with DDP on growth inhibition and apoptosis of lung cancer cell A549
10.7652/jdyxb201703019
- VernacularTitle:重组金属蛋白酶组织抑制因子-3与顺铂联合应用对肺癌细胞A549生长抑制与凋亡的影响
- Author:
Gang LI
;
Hong REN
;
Jiansheng WANG
;
Yunfeng ZHANG
;
Jing ZHANG
;
Ning DU
;
Xin SUN
;
Sida QIN
- Keywords:
lung cancer cell A549;
cell cycle;
apoptosis;
restructuring tissue inhibitor of matrix metalloproteinase-3 (rhTIMP-3);
cisplatin
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(3):422-426
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of restructuring tissue inhibitor of matrix metatloproteinase-3 (rhTIMP-3) in combination with cisplatin (DDP) on the growth and apoptosis of A549 lung cancer cell line.Methods We made individual and combined use of different concentrations of rhTIMP-3 and DDP on A549 cells.Methyl thiazoyl terazolium (MTT) colorimetry was used to analyze cell growth inhibition,and flow cytometry technique was used to determine the cell cycle distribution and apoptosis rate.Results rhTIMP-3 and DDP both could inhibit the proliferation of A549 cells.rhTIMP-3 exerted its effect in the time-and concentration-dependent manners while DDP did so in the concentration-dependent manner;both induced the apoptosis of A549 cells.rhTIMP-3 could make the cells stay in S and G2/M phases,and DDP made the cells stay in S phase.The combination of them obviously strengthened the inhibition of A549 cell growth,and had obvious synergy in inducing apoptosis.Conclusion Both rhTIMP-3 and DDP can inhibit the proliferation of A549 cells and induce their apoptosis.The combined use of them not only can increase the inhibition of cell growth but also has synergy in inducing cell apoptosis.
