Effect of Ulinastatin on the Expression of TNF-α in the Rabbits with Acute Lung Injury Induced by Underwater Explosion
10.3969/j.issn.1671-7414.2017.03.036
- VernacularTitle:乌司他丁干预对水下爆炸致兔急性肺损伤TNF-α表达的影响
- Author:
Fanfan MENG
;
Xiaolin QI
;
Yijiang HUANG
;
Changqing YE
;
Qianjin WANG
;
Honghao MA
;
Xianan SHAO
- Keywords:
underwater explosion;
acute lung injury;
ulinastatin;
tumor necrosis factor-alpha
- From:
Journal of Modern Laboratory Medicine
2017;32(3):131-132,136
- CountryChina
- Language:Chinese
-
Abstract:
Objective The expression of TNF-α was detected in sera of rabbits treated by ulinastatin with acute lung injury in duced by underwater explosion.Methods Rabbits were randomly divided into two groups such as the injured group and ulinastatin therapy group.Established underwater explosion device was used to cause acute lung injury in rabbits.TNF-α in sera of the rabbits were measured by ELISA at 4,12 and 24 hours after the explosion.The SPSS17.0 software was used to analyze the data and P＜0.05 was considered to be significant.Results There was no significant difference between the concentrations of TNF-α in the sera of rabbits in the injure group (538.20±201.43 ng/L) and that of in the ulinastatin group (386.90± 109.22 ng/L,t=2.088,P=0.051) at 4 hours after burst.However,there was evidently decreased in the level of TNF-α in the ulinastatin group (400.60 ± 78.98 ng/L) compared with the injury group (573.80 ± 178.24 ng/L,t =2.809,P =0.012) at 12 hours after burst.Moreover and TNF-α in the ulinastatin group (356.10 ± 130.99 ng/L) was also decreased compared to the injure group (552.30± 169.64 ng/L,t=2.895,P=0.010) at 24 hours after burst.Conclusion The TNF-α expression in sera of the rabbits in ulinastatin group were dramatically decreased than thai of in injury group at 12 hours after burst,which may be benefit to rabbits with acute lung injury induced by underwater explosion.
