Human bone marrow mesenchymal stem cells into vascular endothelial cells before and after differentiation induced differential expression of eNOS/activity and its metabolites
10.7652/jdyxb201703013
- VernacularTitle:人骨髓间充质干细胞定向血管内皮细胞诱导分化前后eNOS表达/活性及其代谢产物的差异
- Author:
Xuelan LUO
;
Wei CHEN
;
Guojun MO
;
Peng YANG
;
Hesheng OU
- Keywords:
human bone marrow mesenchymal stem cells (hBMSCs);
endothelial cell;
induced differentiation;
eNOS;
metabolite
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(3):386-391
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differences in eNOS gene expression,activity and its metabolites before and after human bone marrow mesenchymal stem cells (hBMSCs) are induced into vascular endothelial cells.Methods hBMSCs were induced into vascular endothelial cells.The morphological changes of the cells were observed under inverted microscope.Transwell assay was used to detect the cells' migration ability.The protein expression of eNOS was detected by immunofluorescence and Western blot.The activity of eNOS was detected by ELISA and the content of NO in cell culture supernatant was determined by nitrate reduction method.Results Compared with those in the undifferentiated group,the morphological changes of the differentiated cells were obvious.Cell migration ability increased by 238.10％ (73.000±7.002 vs.21.000±4.359,P＜0.05).The expression of eNOS protein increased by 114.72％ (0.423±0.011 vs.0.197±0.079,P＜0.05).The activity of eNOS was enhanced by 157.49％ (4.967±0.073 vs.1.929±±0.103,P＜0.05).The synthesis and release of NO increased by 155.67％ (184.909±1.853 vs.72.323±0.426,P＜0.05).Conclusion After hBMSCs are induced into endothelial cells,the expression of eNOS gene increases,their activities increase,synthesis and release of the metabolite NO increase.It may provide a basis for prevention and treatment of cardiovascular diseases with stem cells.
