Expression and Its Significance of microRNA-125b and microRNA-133b in Plasma in Children with Asthma
10.3969/j.issn.1671-7414.2017.03.016
- VernacularTitle:儿童支气管哮喘患者血浆中miRNA-125b及miRNA-133b的表达及临床意义
- Author:
Yong LI
;
Chunbiao GAO
;
Weiwu SHENG
;
Hualing GUAN
- Keywords:
athma;
miRNA-125b;
miRNA-133b
- From:
Journal of Modern Laboratory Medicine
2017;32(3):59-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the diagnostic value of the quantitative detection of plasma miRNA-125b and miRNA-133b in children with asthma.Methods Thirty asthmatic patients were enrolled in this study and collected the blood specimens during acute phase and stable phase respectively (AP group and SP group).Thirty allergic rhinitis children (AR group) and thirty healthy children were recruited to the control group (NC group).The levels of miRNA in different groups were detected by qRT-PCR.The performance of miRNA-125b and miRNA-133b were evaluated by receiver operating characteristic curves (ROC) and the area under the curve (AUC) (95％CI).Results The relative expression of miRNA-125b in AP group and SP group were significantly higher than A R group (t=3.913,3.120,P＜0.01),miRNA-133b.In AP group and AR group the expression of miRNA-133b were significantly higher than control group (t=4.426,4.720,P＜0.01).The detection of miRNA-125b yielded an area under the curve of ROC of 0.7989,(95 ％ CI:0.7111～ 0.8864) in discriminating asthmatic patients from healthy group.And the miRNA-133b was 0.7274 (95％CI:0.586 5～0.863 0) in discriminating asthmatic patients during the acute phase from healthy group.Conclusion The relative expression of miRNA-125b in children with asthma was significantly higher than that in AR group and NC group,miRNA-125b may prove to be a non-invasive biomarker for the auxiliary diagnosis of asthma especially when the relative expression up to 1.998.
