Performance Evaluation of Alarms and Parameters Generated by Hematology Analyzer during Detection of Circulating Sezary Cells of Peripheral Blood Smears
10.3969/j.issn.1671-7414.2017.03.025
- VernacularTitle:XN-9000血细胞分析仪筛查外周血中Sezary细胞的临床价值研究
- Author:
Jun HONG
;
Yongcai RAO
- Keywords:
sezary syndrome;
hematology analyzer;
morphology
- From:
Journal of Modern Laboratory Medicine
2017;32(3):92-96
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the performance alarms and parameters generated by hematology analyzer during detection of circulating sezary cells of peripheral blood smears.Methods Blood samples from patients with Sezary syndrome (n=21) were studied with Sysmex XN-9000 analyzer,and compared to patients with benign or tumoral skin lesions (n=48) and patients with chronic lymphoproliferative B-cell diseases (n=55) and normal person (n=45) used as control.Results In present series,the value of structural lymphoid parameters (ly-x and ly-z) and the alarm Blast/Abn Lympho were statistically higher in Sezary cases than in control cases (U=37,40,44,55,38,41,all P＜0.05).Screening Sezary cells critical values of ly-x and ly-z determined by ROC curve were greater than or equal to 86 and was more than or equal to 68.The combination of above three indexes up (i.e.alarm Blast/Abn Lympho＞ 100,ly-x=86 and ly-z＞68) sensitivity and specificity respectively for 100 ％ and 92.1 ％,in order to get the best screening Sezary cells diagnostic efficiency.In addition,the value of ly-x was associated to the count of circulating Sezary cells and value of ly-z to the presence of large Sezary cells,both parameters described as prognostic factors.Conclusion The combination of alarm Blast/Abn Lympho and structural parameters (ly-x/ ly-z/ly-y) may allow to define rule of blood slide review to screen circulating Sezary cells.
