Resistance Mechanisms of Clinical Isolated Carbapenem-Resistant Klebsiella Pneumoniae
10.3969/j.issn.1671-7414.2017.03.030
- VernacularTitle:临床分离的耐碳青霉烯肺炎克雷伯菌的耐药机制研究
- Author:
Li CHENG
;
Tingting TAN
;
Hongxia WEI
;
Kui ZHANG
- Keywords:
carbapenems;
Klebsiella pneumoniae;
drug resistance mechanism
- From:
Journal of Modern Laboratory Medicine
2017;32(3):112-114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecular mechanisms of carbapenem-resistant Klebsiella pneumoniae.Methods Carba NP confirmatory test were used to detect carbapenemases.Carbapenemase genes,ESBL genes and plasmid-mediated AmpC genes were amplified by polymerase chain reaction (PCR).The genetic correlation analysis was carried out by using multiple sequence type (MLST).Results 42 out of the 50 carbapenem-resistant Klebsiella pneumoniae strains were KPC-2-positive strains,1 strain was positive for NDM-1,the other 7 strains were not detected for any carbapenemase genes.The percentages of KPC-2-positive Klebsiella pneumoniae with the bla CTX-M,bla SHV,bla TEM,bla DHA were 21.5 ％,42.9 ％,69.1％ and 4.8％ respectively.The results of MLST showed that 37 out of 42 KPC-2 positive strains were ST11.Conclusion The production of KPC-2 is the main mechanism of Klebsiella pneumoniae resistance to carbapenem and there is an outbreak of ST11 KPC-2 Klebsiella pneumoniae in this hospital.
