Determination of vancomycin in human serum by in situ formed ionic liquids microextraction-ultra performance liquid chromatography (ISFILM-UPLC) methods
10.11665/j.issn.1000-5048.20170210
- VernacularTitle:原位生成离子液体法微萃取-超高效液相色谱法测定万古霉素血药浓度
- Author:
Dejin XU
;
Wenbo YUAN
;
Juan LYU
- Keywords:
in situ formed ionic liquids microextraction;
ultra-performance liquid chromatography;
vancomycin;
plasma concentration;
determination of content
- From:
Journal of China Pharmaceutical University
2017;48(2):196-200
- CountryChina
- Language:Chinese
-
Abstract:
A method based on in situ formed ionic liquids microextraction-ultra performance liquid chromatography(ISFILM-UPLC) was established for the determination of vancomycin in human serum.The samples were pretreated by vortex and centrifugation.[C6MIM] [Br] and NaPF6 were applied as the extracting agent,and Metronidazole as the internal standard.Methanol was applied to redissolve the precipitate.The separation was carried out on a BEH C18 column,and the mobile phase consisted of methanol and 0.05 mol/L monopotassium phosphate solution.The flow rate was 0.2 mL/min,the injection volume was 5 μL,and the column temperature was 18 ℃.The key parameters of this method such as the specificity,accuracy and precision were validated.This method showed good correlation with the immunoassay.It shows great potential for the application in clinical practice.
