Cloning and sequencing of the gene coding for diphtheria toxin from corynebacterium diphtheriae vaccine strain at IVAC
- Author:
Dang Hong Van
- Publication Type:Journal Article
- Keywords:
gen, toxin, diphthria, vaccin
- MeSH:
Diphtheria Toxin;
Genes;
Corynebacterium diphtheriae
- From:Journal of Preventive Medicine
2003;13(6):65-70
- CountryViet Nam
- Language:Vietnamese
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Abstract:
In this study, PCR technique was used with 2 primer pairs for amplifying DNA fragments at 5’ and 3’ of the gene encoding diphteria toxine of the strains Corynebacterium diphtheriae. After the cloning and sequencing, 2 separated fragments were joined to form a complete gene. The sequence of gene was translated into protein and the results were submitted into Gene Bank Database
