Effects of Aloperine on Proliferation and Apoptosis of Bladder Cancer EJ Cells.
10.11969/j.issn.1673-548X.2017.06.012
- VernacularTitle:苦豆碱对膀胱癌EJ细胞增殖与凋亡的影响
- Author:
Cong QIN
;
Jie ZHANG
;
Lijun ZHANG
- Keywords:
Aloperine;
Bladder cancer;
Proliferation;
Apoptosis
- From:
Journal of Medical Research
2017;46(6):44-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of aloperine on the proliferation and apoptosis of bladder cancer EJ cells and to explore its mechanism.Methods;The experimental group was as follows:bladder cancer EJ cells were not treated (group A);25μmol/L aloperine was added (group B);50μmol/L aloperine was added (group C).100μmol/L aloperine was added (group D).The survival rate of the cells was detected by CCK8.The apoptosis rate was detected by flow cytometry.The expression levels of Bcl-2,Bax,p Erk1/2 protein were detected by Western blot.Results CCK8 results showed that:B,C,D group compared with A group,the cell survival rate were decreased (P < 0.05).The results of flow cytometry showed that the apoptosis rate of B,C and D group was higher than that of A group (P < 0.05).Western blot results showed that:B,C,D group compared with A group the expression of Bcl-2 and p Erk1/2 levels were reduced (P < 0.05),the expression of Bax level was increased (P < 0.05).Conclusion The survival rate of EJ in bladder cancer can be reduced by aloperine and induce its apoptosis,and inhibiting the phosphorylation of Erk1/2 may be the mechanism.