Role of lupus murine B cells in abnormal development of T cell subsets
10.3760/cma.j.issn.1007-7480.2017.08.002
- VernacularTitle:狼疮性小鼠B细胞促进T细胞亚群的异常分化发育
- Author:
Jiyu JU
;
Zhiwei XU
- Keywords:
Lupus erythematosus,systemic;
Mouse model;
Receptors,antigen,B-cell;
T-lymphocyte cell subsets;
Differentiation
- From:Chinese Journal of Rheumatology
2017;21(8):508-512
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of B cells from lupus prone Triple congenic (TC) mouse model on the differentiation and development of T cell subsets. Methods The spleen size and B cell numbers were measured, and surface CD40, CD86 and Ⅰ-Ab molecules on B cells as well as CD4+T cell subsets were detected using flow cytometry when the spontaneous systemic lupus erythematosus (SLE) model TC mice and control B6 mice were 6 months old. In addition, the chimera of TC B cells and B6 CD4+T cells or chimera of B6 B cells and B6 CD4+ T cells were transferred into B6.Rag-/- mice via intravenous injection. Then, T cell subsets in the spleen of recipient B6.Rag-/-mice were observed 7 days after cell transplantation. Results TC mice had significantly bigger spleen [(5337±934) mg] and more CD19+B cell number [(276.0±48.7)×107] than control B6 mice [spleen weight: (91±4) mg; B cell number: (6.4±0.3)×107](P<0.01). TC mice showed markedly increased CD40 [MFI (63.6±3.1)], CD86 [(MFI (18.96±0.44)] and Ⅰ-Ab [MFI (637±41) on spleen B cells compared with that of B6 mice [CD40 MFI: (36.6 ±2.0); CD86 MFI: (14.26 ±0.19); Ⅰ-Ab MFI: (307 ±23)] (P<0.01). In addition, TC mice revealed notably more Th1 subset [(36.54 ±4.22)%] in spleen than B6 mice [Th1 subset: (19.90±0.10)%] [P<0.01], but both strains had equivalent percentages of Th17 and IL-21+CD4+T cell populations (P>0.05). The recipient B6.Rag-/-mice transplanted with TC B cells had significantly more Th1 subset [(54.1±2.8)%] and IL-21+CD4+T cell population [(14.3±1.0)%], but less Th17 subset [(2.05±0.09)%] in spleen than the recipient B6.Rag-/-mice administered by B6 B cells [Th1 subset: (39.5±1.1)%; IL-21+CD4+T cell population:(7.5±1.2)%;Th17 subset:(6.45±1.10)%](P<0.01). Conclusion The B cells of lupus-prone TC mice exhibit a markedly hyper-activation in spleen, and promote CD4+T cells differentiation preferentially into Th1 subset and IL-21+CD4+T cell population, which may further contribute to SLE pathogenesis.
