Whole-genome analysis for 32 influenza A(H3N2) viruses in severe acute respiratory infection cases

VernacularTitle:32例甲型H3N2流感病毒感染严重急性呼吸道感染病例病毒全基因组分析
Author: Yang PAN ; Yi ZHANG ; Peng YANG ; Weixian SHI ; Xiaomin PENG ; Shujuan CUI ; Daitao ZHANG ; Guilan LU ; Jiachen ZHAO ; Quanyi WANG
Keywords: influenza A virus; vaccine; genome; phylogenesis; variation
From: International Journal of Laboratory Medicine 2017;38(17):2367-2370
CountryChina
Language:Chinese
Abstract: Objective To investigate the genetic characteristic of whole-genome of influenza A/H3N2 viruses in severe acute respiratory infection cases in Beijing area.Methods From 2014 to 2016,the viral RNA was extracted from 32 strains isolated from SARI cases,then sequenced by Ion Torrent PGM Sequencer.The phylogeny and molecular features of whole-genome were analyzed by Mega and Consurf software.Results The HA gene of tested strains isolated in 2014-2015 influenza season belonged to lineage 3C.3a and 3C.2a,while those isolated in 2015-2016 influenza season belonged to cluster 3C.2a.Moreover,compared with the vaccine strains,7 variant amino acids of protein of HA1 were identified,and two of them were located in antigenic sites.All isolates were sensitive to neuraminidase inhibitors while showed resistance to blockers for M2 ion channel.Conclusion The phylogenetic features of isolates studied in this study are similar with that of current circulating strains.However,the difference between isolates and vaccines should not be overlooked.