Compare the results of HER-2 immunostaining among different clone numbers and with the results of FISH detection in breast cancer
10.3969/j.issn.1673-4130.2017.17.009
- VernacularTitle:乳腺癌中不同克隆号HER-2抗体IHC与FISH检测结果比较
- Author:
Aiju FANG
;
Xiaoying ZHANG
;
Fengfeng CHENG
;
Zongyan DAI
;
Jing YU
- Keywords:
breast cancer;
HER-2 antibody;
clone;
fluorescence in situ hybridization
- From:
International Journal of Laboratory Medicine
2017;38(17):2357-2359
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the immunostaining of human epidermal growth factor receptor-2(HER-2) among three different clones,and to compare with the results of fluorescence in situ hybridization(FISH) detection,to provide evidences for pathological department to select appropriate antibodies.Methods 268 cases of invasive breast cancer were examined by immunohistochemistry using antibodies for HER-2 of 3 different clones.The results were compared with those done by FISH.Results Immunostaining using antibody clone SP3 showed 3+ reactions in 66 cases;Immunostaining using antibody clone EP3 showed 3+ reactions in 53 cases,while using polyclonal HER-2 antibody,80 cases showed 3+ reactions.There was statistical significance between those groups using different clones(P<0.05).Consistent with the FISH results,the Kappa values are 0.76,0.67,and 0.56,respectively.Conclusion It suggests detection of HER-2 using immunohistochemistry should prioritize selecting monoclonal antibodies,especially clone SP3.
