The analysis of interference factors and solutions to the cross contaminationbetween total bile acid and 30 inspection items on the Roche biochenical analyer
10.3969/j.issn.1673-4130.2017.17.025
- VernacularTitle:罗氏生化分析仪上30个检验项目对总胆汁酸测定的干扰因素分析及解决措施
- Author:
Qiuhua JIA
- Keywords:
high density lipoprotein;
uric acid;
lipase;
total bile acid;
cross contamination
- From:
International Journal of Laboratory Medicine
2017;38(17):2404-2405,2408
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the interference factors of total bile acid project and 30 routine items on the Roche Modular P800 automatic biochemical analyzer,and to find the effective measures to eliminate the carry-over.Methods In accordance with the screening process,the interference factors of total bile acid project as the test item,30 regular testing projects as the observation objects,including total bilirubin,direct bilirubin and so on,and then the validation test were carried out.By means of the increasing cleaning procedures of the reagent needles or colorimetric cups and adjusting the test sequence,we compared the influence of the result of total bile acid before and after this kind of treatment.Results There were the carry-over of high density lipoprotein,uric acid and total bile acid,which could be eliminated by increasing cleaning procedures of reagent needles or colorimetric cups.There were the cross contamination of lipase and total bile acid,which could be basically eliminated by adjusting the test sequence.Conclusion There are the cross contamination among the various projects of automatic biochemical analyzer.To avoid this carry-over,we should pay attention to the order of the infectant and contaminated items in setting the analysis parameters,insert several projects in the interval,or add the cleaning procedures of the reagent needles or colorimetric cups with alkali or acid.
