Changes of ionized calcium binding adapter molecule 1 and pro-opiome-lanocortin expression in arcuate nucleus of hypothalamus induced by substantia nigra lesion with 6-hydroxydopamine
10.3969/j.issn.1000-4718.2017.06.003
- VernacularTitle:6-羟多巴损伤黑质导致弓状核离子钙接头蛋白1和阿片-促黑素皮质素原表达的变化
- Author:
Yimin ZHANG
;
Hui LIAN
;
Haiyan SONG
;
Li ZHOU
;
Junyan CAI
;
Zhiyong WANG
- Keywords:
6-Hydroxydopamine;
Arcuate nucleus;
Tyrosine hydroxylase;
Ionized calcium binding adapter molecule 1;
Pro-opiomelanocortin
- From:
Chinese Journal of Pathophysiology
2017;33(6):975-980
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the expression of tyrosine hydroxylase (TH), ionized calcium binding adapter molecule 1 (Iba1) and pro-opiomelanocortin (POMC) in rat arcuate nucleus (Arc) of hypothalamus induced by substantia nigra (SN) lesion with 6-hydroxydopamine (6-OHDA).METHODS:According to the random number table, 20 SD rats were randomly divided into 6-OHDA group and control group.6-OHDA was injected into the bilateral SN of the rats in 6-OHDA group, and the same volume of saline was injected into the same position of the rats in control group.After 6 weeks, the rats were sacrificed and the brains were obtained.Immunofluorescence staining and Western blot were used to detect TH expression in the SN, and TH, Iba1 and POMC expression in the Arc.RESULTS:Compared with control group, no significant change of body weight in 6-OHDA group was observed, but the weight of retroperitoneal fat decreased from (7.550±0.670) g to (3.895±0.465) g (P<0.01).The number of TH immunoreactive neurons in SN decreased from 56±5 to 14±2 (P<0.05), and the TH protein level decreased from 0.75±0.11 to 0.41±0.09 (P<0.01).In the Arc, the number of TH immunoreactive neurons decreased from 21±2 to 10±3 (P<0.05), the number of Iba1 immunoreactive neurons increased from 12±2 to 30±5 (P<0.05), and the number of POMC immunoreactive neurons increased from 16±4 to 31±2 (P<0.05).In the hypothalamus, the TH protein level decreased from 0.35±0.05 to 0.21±0.02 (P<0.01), the Iba1 protein level increased from 0.23±0.06 to 0.51±0.04 (P<0.01), and the POMC protein level increased from 0.37±0.05 to 0.65±0.03 (P<0.01).CONCLUSION:The changes of TH, Iba1 and POMC expression in Arc of 6-OHDA-treated rats may involved in the fat loss in Parkinson's disease.
