Effect of amygdala H2S system on depression-like behavior in posttraumatic stress disorder rats
10.3969/j.issn.1000-4718.2017.06.005
- VernacularTitle:杏仁核脑区硫化氢对创伤后应激障碍模型大鼠抑郁样行为的影响
- Author:
Hongxia LIU
;
Hong PAN
;
Hua WANG
;
Shunjun JIANG
;
Zhang CAO
;
Wenyu XIN
- Keywords:
Hydrogen sulfide;
Posttraumatic stress disorder;
Cystathionine β-synthase;
Amygdala
- From:
Chinese Journal of Pathophysiology
2017;33(6):988-992
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the depression-like behavior and cystathionine β-synthase (CBS)/hydrogen sulfide (H2S) levels of amygdala in posttraumatic stress disorder (PTSD) rats and to study the effect of exogenous H2S on PTSD rats.METHODS:Single prolonged stress paradigm was adopted to prepare PTSD animal model.Forced swimming test and sucrose preference test were used to evaluate the depression-like behavior.The content of CBS/H2S in amygdala tissue was measured by Western blot and methylene blue method.In vivo extracellular single unit recordings was used to examine the frequency of spontaneous discharges of amygdala neurons.RESULTS:The immobility time in forced swimming test of PTSD group increased and sucrose preference in sucrose preference test of PTSD group decreased compare with normal group (P<0.01).CBS/H2S level in amygdala tissue of PTSD group decreased compared with normal group (P<0.01).The immobility time of the rats in forced swimming test of NaHS+PTSD group decreased and the sucrose prefe-rence in sucrose preference test of NaHS+PTSD group increased compare with PTSD group (P<0.01).L-cysteine increased the frequency of spontaneous discharges of amygdala neurons (P<0.01).CONCLUSION:Depression-like behavior is aggravated in PTSD model rats owing to the inhibition of CBS/H2S content in amygdala tissue.The mechanism of behavior-improving effect of H2S on PTSD model rats is possibly related to excitating amygdala neurons and increasing the frequency of spontaneous discharges.
