STC2 promotes proliferation and EMT-related migration of HepG2 cells
10.3969/j.issn.1000-4718.2017.06.007
- VernacularTitle:STC2促进人肝癌细胞HepG2增殖和EMT相关的迁移
- Author:
Lei CAO
;
Lei LI
;
Mingzhu HU
;
Yimei ZHANG
;
Haihua LUO
;
Shuiwang HU
;
Aihua LIU
;
Yong JIANG
- Keywords:
Hepatocellular carcinoma;
Stanniocalcin 2;
Epithelial-mesenchymal transition;
Cell migration
- From:
Chinese Journal of Pathophysiology
2017;33(6):1000-1005
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the effects of stanniocalcin 2 (STC2) on the proliferation, migration and the process of epithelial-mesenchymal transition (EMT) in human hepatocellular carcinoma HepG2 cells.METHODS:The expression levels of STC2 in the hepatocellular carcinoma cell lines and normal liver cells were assessed by Western blot.Colony formation assay was used to test the effect of STC2 on the proliferation of HepG2 cells.The effects of STC2 on the expression of proliferation-related molecules at mRNA and protein levels were determined by RT-qPCR and Western blot.The effect of STC2 on the migration ability was measured by Transwell assay.The mRNA and protein levels of vimentin and E-cadherin in STC2-overexpressing and-silencing cell lines were detected by RT-qPCR and Western blot.RESULTS:Compared with the normal liver cell line, the protein expression of STC2 was up-regulated in the hepatocellular carcinoma cell lines.The results of colony formation assay indicated that STC2 promoted the proliferation of HepG2 cells.STC2 significantly regulated the proliferation-related gene expression, such as cyclin D1.The results of Transwell assay showed that STC2 enhanced the migration ability of the HepG2 cells and influenced the EMT process.CONCLUSION:STC2 promotes the proliferation of HepG2 cells and affects the expression of proliferation-related genes.STC2 influences the process of EMT and promotes the migration of HepG2 cells.
