Effects of VDUP1 on proliferation and migration of human breast cancer MCF-7 cells
10.3969/j.issn.1000-4718.2017.06.017
- VernacularTitle:VDUP1对乳腺癌细胞MCF-7增殖及迁移的影响
- Author:
Jianhua LI
;
Liqiang XU
;
Xiuwen NI
;
Yayun SUN
;
Huina MAO
;
Jinhui WU
- Keywords:
Vitamin D3 up-regulated protein 1;
MCF-7 cells;
Cell proliferation;
Cell migration;
Akt/GSK3β pathway
- From:
Chinese Journal of Pathophysiology
2017;33(6):1060-1064
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of vitamin D3 up-regulated protein 1 (VDUP1) gene over-expression/knockdown on the proliferation and migration of human breast cancer MCF-7 cells and its related mechanisms.METHODS:Gene over-expression/interference techniques were used to up-regulate/down-regulate the expression of VDUP1 in the MCF-7 cells.The mRNA expression of VDUP1 was detected by qPCR.CCK-8, BrdU and Transwell assays were used to measure the cell viability, proliferation and migration, respectively.The protein levels of Akt, p-Akt, GSK3β and p-GSK3β were determined by Western blot.RESULTS:The mRNA expression of VDUP1 was up-regulated after transfection with VDUP1 over-expression plasmid (P<0.05), and down-regulated after transfection with VDUP1 siRNA (P<0.05).Over-expression of VDUP1 significantly inhibited MCF-7 cell proliferation and migration (P<0.05), while knockdown of VDUP1 enhanced cell proliferation and migration (P<0.05).Furthermore, over-expression of VDUP1 up-regulated the protein levels of p-Akt and p-GSK3β (P<0.05).Inverse results were obtained after knockdown of VDUP1.CONCLUSION:The viability and migration ability of MCF-7 cells are inhibited by over-expression of VDUP1 but enhanced by VDUP1 knockdown, which may be related with Akt/GSK3β pathway.
